Epidemiological Investigations and Molecular Characterization of 'Candidatus Phytoplasma solani' in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily, (Southern Italy).
Identifieur interne : 000071 ( Main/Exploration ); précédent : 000070; suivant : 000072Epidemiological Investigations and Molecular Characterization of 'Candidatus Phytoplasma solani' in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily, (Southern Italy).
Auteurs : Gaetano Conigliaro [Italie] ; Elham Jamshidi [Italie] ; Gabriella Lo Verde [Italie] ; Patrizia Bella [Italie] ; Vincenzo Mondello [France] ; Selene Giambra [Italie] ; Vera D'Urso [Italie] ; Haralabos Tsolakis [Italie] ; Sergio Murolo [Italie] ; Santella Burruano [Italie] ; Gianfranco Romanazzi [Italie]Source :
- Pathogens (Basel, Switzerland) [ 2076-0817 ] ; 2020.
Abstract
Bois noir is caused by 'Candidatus Phytoplasma solani', and it is one of the most important and widespread diseases in the Euro-Mediterranean region. There are complex interactions between phytoplasma and grapevines, weeds, and vectors. These ecological relationships can be tracked according to molecular epidemiology. The aims of the 2-year study (2014-2015) were to describe incidence and spatial distribution of Bois noir in a vineyard with three grapevine varieties in Sicily, and to identify the molecular types of the tuf and vmp1 genes in these naturally infected grapevines, according to the potential reservoir plants and vectors. Disease incidence in 2015 was significantly higher in 'Chardonnay' (up to 35%) than for 'Nero d'Avola' and 'Pinot noir' (<5%). All grapevine, weed, and insect samples were infected by 'Ca. P. solani' tuf-type b. Most of the collected insects were strictly related to Vitis spp. and belonged to Neoaliturus fenestratus, Empoasca spp., and Zygina rhamni. The characterization of the vmp1 gene revealed six different vmp types in grapevines (V1, V4, V9, V11, V12, V24), three in weeds (V4, V9, V11), and four in insects (V4, V9, V11, V24). Notably, V4, V9, appear both in hosts and vectors, with V9 predominant. Virtual restriction fragment length polymorphism (RFLP) analysis based on the nucleotide sequences supported the data of the conventional RFLP. Connections between the molecular data recorded in the vineyard ecosystems and the application of innovative tools based on the geostatistical analysis will contribute to further clarification of the specific ecological and epidemiological aspects of 'Ca. P. solani' in Sicily.
DOI: 10.3390/pathogens9110918
PubMed: 33171934
Affiliations:
- France, Italie
- Champagne-Ardenne, Grand Est, Sicile
- Palerme, Reims
- Université de Palerme, Université de Reims Champagne-Ardenne
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sort="Conigliaro, Gaetano" uniqKey="Conigliaro G" first="Gaetano" last="Conigliaro">Gaetano Conigliaro</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="Jamshidi, Elham" sort="Jamshidi, Elham" uniqKey="Jamshidi E" first="Elham" last="Jamshidi">Elham Jamshidi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona</wicri:regionArea><wicri:noRegion>60131 Ancona</wicri:noRegion></affiliation></author><author><name sortKey="Lo Verde, Gabriella" sort="Lo Verde, Gabriella" uniqKey="Lo Verde G" first="Gabriella" last="Lo Verde">Gabriella Lo Verde</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="Bella, Patrizia" sort="Bella, Patrizia" uniqKey="Bella P" first="Patrizia" last="Bella">Patrizia Bella</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="Mondello, Vincenzo" sort="Mondello, Vincenzo" uniqKey="Mondello V" first="Vincenzo" last="Mondello">Vincenzo Mondello</name><affiliation wicri:level="4"><nlm:affiliation>Résistance Induite et Bioprotection des Plantes (RIBP), SFR Condorcet FR CNRS 3417, Université de Reims Champagne-Ardenne, EA 4707, BP 1039, CEDEX 2, 51687 Reims, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Résistance Induite et Bioprotection des Plantes (RIBP), SFR Condorcet FR CNRS 3417, Université de Reims Champagne-Ardenne, EA 4707, BP 1039, CEDEX 2, 51687 Reims</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author><author><name sortKey="Giambra, Selene" sort="Giambra, Selene" uniqKey="Giambra S" first="Selene" last="Giambra">Selene Giambra</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="D Urso, Vera" sort="D Urso, Vera" uniqKey="D Urso V" first="Vera" last="D'Urso">Vera D'Urso</name><affiliation wicri:level="1"><nlm:affiliation>Sezione di Biologia Animale, Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università degli Studi di Catania, via Androne 81, 95124 Catania, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Sezione di Biologia Animale, Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università degli Studi di Catania, via Androne 81, 95124 Catania</wicri:regionArea><wicri:noRegion>95124 Catania</wicri:noRegion></affiliation></author><author><name sortKey="Tsolakis, Haralabos" sort="Tsolakis, Haralabos" uniqKey="Tsolakis H" first="Haralabos" last="Tsolakis">Haralabos Tsolakis</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="Murolo, Sergio" sort="Murolo, Sergio" uniqKey="Murolo S" first="Sergio" last="Murolo">Sergio Murolo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona</wicri:regionArea><wicri:noRegion>60131 Ancona</wicri:noRegion></affiliation></author><author><name sortKey="Burruano, Santella" sort="Burruano, Santella" uniqKey="Burruano S" first="Santella" last="Burruano">Santella Burruano</name><affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo</wicri:regionArea><orgName type="university">Université de Palerme</orgName><placeName><settlement type="city">Palerme</settlement><region type="region" nuts="2">Sicile</region></placeName></affiliation></author><author><name sortKey="Romanazzi, Gianfranco" sort="Romanazzi, Gianfranco" uniqKey="Romanazzi G" first="Gianfranco" last="Romanazzi">Gianfranco Romanazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona</wicri:regionArea><wicri:noRegion>60131 Ancona</wicri:noRegion></affiliation></author></analytic><series><title level="j">Pathogens (Basel, Switzerland)</title><idno type="ISSN">2076-0817</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bois noir is caused by '<i>Candidatus</i> Phytoplasma solani', and it is one of the most important and widespread diseases in the Euro-Mediterranean region. There are complex interactions between phytoplasma and grapevines, weeds, and vectors. These ecological relationships can be tracked according to molecular epidemiology. The aims of the 2-year study (2014-2015) were to describe incidence and spatial distribution of Bois noir in a vineyard with three grapevine varieties in Sicily, and to identify the molecular types of the <i>tuf</i> and <i>vmp</i>1 genes in these naturally infected grapevines, according to the potential reservoir plants and vectors. Disease incidence in 2015 was significantly higher in 'Chardonnay' (up to 35%) than for 'Nero d'Avola' and 'Pinot noir' (<5%). All grapevine, weed, and insect samples were infected by '<i>Ca.</i> P. solani' <i>tuf</i>-type b. Most of the collected insects were strictly related to <i>Vitis</i> spp. and belonged to <i>Neoaliturus fenestratus</i>, <i>Empoasca</i> spp., and <i>Zygina rhamni</i>. The characterization of the <i>vmp</i>1 gene revealed six different <i>vmp</i> types in grapevines (V1, V4, V9, V11, V12, V24), three in weeds (V4, V9, V11), and four in insects (V4, V9, V11, V24). Notably, V4, V9, appear both in hosts and vectors, with V9 predominant. Virtual restriction fragment length polymorphism (RFLP) analysis based on the nucleotide sequences supported the data of the conventional RFLP. Connections between the molecular data recorded in the vineyard ecosystems and the application of innovative tools based on the geostatistical analysis will contribute to further clarification of the specific ecological and epidemiological aspects of '<i>Ca.</i> P. solani' in Sicily.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33171934</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>11</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-0817</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>06</Day></PubDate></JournalIssue><Title>Pathogens (Basel, Switzerland)</Title><ISOAbbreviation>Pathogens</ISOAbbreviation></Journal><ArticleTitle>Epidemiological Investigations and Molecular Characterization of '<i>Candidatus</i> Phytoplasma solani' in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily, (Southern Italy).</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E918</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/pathogens9110918</ELocationID><Abstract><AbstractText>Bois noir is caused by '<i>Candidatus</i> Phytoplasma solani', and it is one of the most important and widespread diseases in the Euro-Mediterranean region. There are complex interactions between phytoplasma and grapevines, weeds, and vectors. These ecological relationships can be tracked according to molecular epidemiology. The aims of the 2-year study (2014-2015) were to describe incidence and spatial distribution of Bois noir in a vineyard with three grapevine varieties in Sicily, and to identify the molecular types of the <i>tuf</i> and <i>vmp</i>1 genes in these naturally infected grapevines, according to the potential reservoir plants and vectors. Disease incidence in 2015 was significantly higher in 'Chardonnay' (up to 35%) than for 'Nero d'Avola' and 'Pinot noir' (<5%). All grapevine, weed, and insect samples were infected by '<i>Ca.</i> P. solani' <i>tuf</i>-type b. Most of the collected insects were strictly related to <i>Vitis</i> spp. and belonged to <i>Neoaliturus fenestratus</i>, <i>Empoasca</i> spp., and <i>Zygina rhamni</i>. The characterization of the <i>vmp</i>1 gene revealed six different <i>vmp</i> types in grapevines (V1, V4, V9, V11, V12, V24), three in weeds (V4, V9, V11), and four in insects (V4, V9, V11, V24). Notably, V4, V9, appear both in hosts and vectors, with V9 predominant. Virtual restriction fragment length polymorphism (RFLP) analysis based on the nucleotide sequences supported the data of the conventional RFLP. Connections between the molecular data recorded in the vineyard ecosystems and the application of innovative tools based on the geostatistical analysis will contribute to further clarification of the specific ecological and epidemiological aspects of '<i>Ca.</i> P. solani' in Sicily.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Conigliaro</LastName><ForeName>Gaetano</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0001-5670-5286</Identifier><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jamshidi</LastName><ForeName>Elham</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lo Verde</LastName><ForeName>Gabriella</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0001-8207-1416</Identifier><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bella</LastName><ForeName>Patrizia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mondello</LastName><ForeName>Vincenzo</ForeName><Initials>V</Initials><Identifier Source="ORCID">0000-0003-4005-0494</Identifier><AffiliationInfo><Affiliation>Résistance Induite et Bioprotection des Plantes (RIBP), SFR Condorcet FR CNRS 3417, Université de Reims Champagne-Ardenne, EA 4707, BP 1039, CEDEX 2, 51687 Reims, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Giambra</LastName><ForeName>Selene</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>D'Urso</LastName><ForeName>Vera</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Sezione di Biologia Animale, Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università degli Studi di Catania, via Androne 81, 95124 Catania, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsolakis</LastName><ForeName>Haralabos</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0001-8018-9496</Identifier><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murolo</LastName><ForeName>Sergio</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-7269-1734</Identifier><AffiliationInfo><Affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burruano</LastName><ForeName>Santella</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Viale delle Scienze Ed. 4, 90128 Palermo, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Romanazzi</LastName><ForeName>Gianfranco</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0003-0390-4008</Identifier><AffiliationInfo><Affiliation>Department of Agricultural, Food and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>Project: 2017-NAZ-0228 - CUP B78117000260008</GrantID><Agency>Ministero Sviluppo Economico</Agency><Country></Country></Grant><Grant><GrantID>314</GrantID><Agency>Marche Polytechnic University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Pathogens</MedlineTA><NlmUniqueID>101596317</NlmUniqueID><ISSNLinking>2076-0817</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">grapevine</Keyword><Keyword MajorTopicYN="N">grapevine yellows</Keyword><Keyword MajorTopicYN="N">phytoplasma</Keyword><Keyword MajorTopicYN="N">tuf gene</Keyword><Keyword MajorTopicYN="N">vectors</Keyword><Keyword MajorTopicYN="N">vmp1 gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>11</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>1</Hour><Minute>2</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33171934</ArticleId><ArticleId IdType="pii">pathogens9110918</ArticleId><ArticleId IdType="doi">10.3390/pathogens9110918</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>Italie</li></country><region><li>Champagne-Ardenne</li><li>Grand Est</li><li>Sicile</li></region><settlement><li>Palerme</li><li>Reims</li></settlement><orgName><li>Université de Palerme</li><li>Université de Reims Champagne-Ardenne</li></orgName></list><tree><country name="Italie"><region name="Sicile"><name sortKey="Conigliaro, Gaetano" sort="Conigliaro, Gaetano" uniqKey="Conigliaro G" first="Gaetano" last="Conigliaro">Gaetano Conigliaro</name></region><name sortKey="Bella, Patrizia" sort="Bella, Patrizia" uniqKey="Bella P" first="Patrizia" last="Bella">Patrizia Bella</name><name sortKey="Burruano, Santella" sort="Burruano, Santella" uniqKey="Burruano S" first="Santella" last="Burruano">Santella Burruano</name><name sortKey="D Urso, Vera" sort="D Urso, Vera" uniqKey="D Urso V" first="Vera" last="D'Urso">Vera D'Urso</name><name sortKey="Giambra, Selene" sort="Giambra, Selene" uniqKey="Giambra S" first="Selene" last="Giambra">Selene Giambra</name><name sortKey="Jamshidi, Elham" sort="Jamshidi, Elham" uniqKey="Jamshidi E" first="Elham" last="Jamshidi">Elham Jamshidi</name><name sortKey="Lo Verde, Gabriella" sort="Lo Verde, Gabriella" uniqKey="Lo Verde G" first="Gabriella" last="Lo Verde">Gabriella Lo Verde</name><name sortKey="Murolo, Sergio" sort="Murolo, Sergio" uniqKey="Murolo S" first="Sergio" last="Murolo">Sergio Murolo</name><name sortKey="Romanazzi, Gianfranco" sort="Romanazzi, Gianfranco" uniqKey="Romanazzi G" first="Gianfranco" last="Romanazzi">Gianfranco Romanazzi</name><name sortKey="Tsolakis, Haralabos" sort="Tsolakis, Haralabos" uniqKey="Tsolakis H" first="Haralabos" last="Tsolakis">Haralabos Tsolakis</name></country><country name="France"><region name="Grand Est"><name sortKey="Mondello, Vincenzo" sort="Mondello, Vincenzo" uniqKey="Mondello V" first="Vincenzo" last="Mondello">Vincenzo Mondello</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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